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[koraltptp]

Hi All,
First time, I will do Column Chromatography for eluting Acrylonitrile from its inhibitor MeHQ.  I have some questions coming to my mind to be sure I do this correctly.

 

I will use activated alumina as the stationary phase, and pour a slurry of Alumina in column.  Then,  Let’s say I have a solution of 10 g Acrylonitrile in 50 ml solvent.  I pour the solution in column, and open the valve, and let some solution to flow down.  Simultaneously, I will add more solvent from top, and continue to collect liquid from bottom.

 

- Should I finish this process until no solvent remains in stationary phase?
- or Should I leave some solvent covering the stationary phase?
- After this process, can I collect %100 of the 10 gr Acrylonitrile at bottom, or will be any loss in stationary phase?

 

As I guess, if I leave some solvent with stationary phase, there will be some amount of Acrylonitrile dissolved in the solvent there.  So, this can cause a small fraction of Acrylonitrile loss.

 

- If I let all the solvent to flow out (no liquid remains in the stationary phase), will any MeHQ inhibitor flow out with solvent? ....or does MeHQ always sticks to the Alumina in column?

 

I would appreciate your answers.

Regards.